- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-11350b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine E3 ubiquitin-protein ligase ARIH1,ARIH1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine E3 ubiquitin- protein ligase ARIH1, ARIH1 ELISA KIT
Product Name:Bovine E3 ubiquitin- protein ligase ARIH1, ARIH1 ELISA KIT
Packing:96T
Catalog No.:ELI-11350b
Gene Name:Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1
Alternative Name:ARIH1,Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine E3 ubiquitin- protein ligase ARIH1, ARIH1 ELISA KIT allows for the in vitro quantitative determination of Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine E3 ubiquitin- protein ligase ARIH1, ARIH1 ELISA KIT has been pre-coated with an Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1 antibody specific to Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine E3 ubiquitin-protein ligase ARIH1, ARIH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
同天两篇 Science，揭晓 miRNA 靶向降解机制，解了悬而未决近 30 年的谜题
作用机制。尽管人类早已明确 miRNA 半衰期非常短，细胞在使用完毕后如何处理（降解）miRNA，精确调控 miRNA 周转机制的蛋白和执行策略知之甚少。 近三十多年来，研究人员一直在寻找细胞降解 miRNAs 的机制。近日，Science 杂志同期发表了两篇关于细胞降解 miRNAs 新机制的重磅研究成果。 德克萨斯大学西南医学中心分子生物学系 Joshua T. Mendell 教授研究团队发现了一种细胞降解 miRNAs 的新机制。该成果以 A ubiquitin ligase mediates
Overview of Protein Microarrays
gluconeogenesis. Cell 136:1073‐1084. Lu, J.‐Y., Lin, Y.‐Y., Qian, J., Tao, S.‐C., Zhu, J., Pickart, C., and Zhu, H. 2008. Functional dissection of a HECT ubiquitin E3 Ligase. Mol. Cell
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