- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-06526b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Creatine kinase B-type,CKB
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Creatine kinase B- type, CKB ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Creatine kinase B-type,B-CK,Creatine kinase B chain, creatine kinase, brain; creatine kinase-B; creatine kinase B-type; creatine kinase B
chain; B-CK; CKBB,CKB
Search name
Bovine Creatine kinase B-type ELISA KIT ,Bovine B-CK ELISA KIT ,Bovine Creatine kinase B chain ELISA KIT ,Bovine creatine kinase ELISA KIT ,Bovine brain ELISA KIT ,Bovine creatine kinase-B ELISA KIT ,Bovine creatine kinase B-type ELISA KIT ,Bovine creatine kinase B chain ELISA KIT ,Bovine B-CK ELISA KIT ,Bovine CKBB ELISA KIT ,Bovine CKB ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine creatine kinase b-type,Creatine kinase B-type
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine kinase B-type. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Creatine kinase
B-type and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain Creatine kinase B-type, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Creatine kinase B-type in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
3) Day 1: Replace medium and culture cells a. 24 hours after transduction, replace the medium with fresh fibroblast medium. b. Note: Depending on your cell type, you should expect to see some cytotoxicity 24–48 hours post
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
. Hutti, J.E., Jarrell, E.T., Chang, J.D., Abbott, D.W., Storz, P., Toker, A., Cantley, L.C., and Turk, B.E. 2004. A rapid method for determining protein kinase phosphorylation specificity. Nat. Methods 1:27‐29
Identification of a Mutant Kinase/ATP Analog Pair
a "pocket" in the ATP-binding site that allows the mutant kinase, but not the wild-type kinase or other cellular kinases, to utilize analogs of ATP with bulky substituents synthesized onto the N6 position. Kinase reactions performed with a mutated kinase
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