- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-06128b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Aggrecan core protein,ACAN
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Aggrecan core protein, ACAN ELISA KIT
Product Name:Bovine Aggrecan core protein, ACAN ELISA KIT
Packing:96T
Catalog No.:ELI-06128b
Gene Name:Bovine Aggrecan core protein, ACAN
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Aggrecan core protein, ACAN
Alternative Name:ACAN,Bovine Aggrecan core protein, ACAN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Aggrecan core protein, ACAN ELISA KIT allows for the in vitro quantitative determination of Bovine Aggrecan core protein, ACAN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Aggrecan core protein, ACAN ELISA KIT has been pre-coated with an Bovine Aggrecan core protein, ACAN antibody specific to Bovine Aggrecan core protein, ACAN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Aggrecan core protein, ACAN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Aggrecan core protein, ACAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Aggrecan core protein, ACAN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验容纳更多的蛋白，并且能将信号放大。具有半定量的特点。 Available kits： Clontech的 TransFactor STAT3 Colorimetric Kit (1 x 96 assays, Catalog No. 631953, $583), Panomics的 HIF ELISA Kit (1 x 96 assays, Catalog No. EK1020, $518), Active Motif的 TransAM CREB Kit (1 x 96
Antigen Design Sera Purification Tech Sheet
to recognize that the immune system reacts to the peptide-protein carrier as a whole and that there will be a portion of response directed against the conjugated peptide as well as the linker and the carrier protein1. When screening by ELISA it is advisable
tag on either the sense or antisense strand (City of Hope Protein and synthesis core facility) and various iterations were generated by first heating the RNAs (sense or antisense +/- biotin) to 70°C for 5 minutes and then mixed together and room
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