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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Cyclin-dependent kinase 1,CDK1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Cyclin- dependent kinase 1, Cdk1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC2,cell cycle controller CDC2; CDC28A; P34CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase;CDK1,CDC2;CDC28A;CDKN1;P34CDC2,Cyclin-dependent kinase 1,CDK1, p34 protein kinase;Cell division control protein 2 homolog;Cyclin-dependent kinase inhibitor 1,p21,CDK-interacting protein 1,Melanoma differentiation-associated protein,
Search name
Bovine CDC2 ELISA KIT ,Bovine cell cycle controller CDC2 ELISA KIT ,Bovine CDC28A ELISA KIT ,Bovine P34CDC2 ELISA KIT ,Bovine cell division control protein 2 homolog ELISA KIT ,Bovine cell division cycle 2 ELISA KIT ,Bovine G1 to S and G2 to M ELISA KIT ,Bovine cell division protein kinase 1 ELISA KIT ,Bovine p34 protein kinase;CDK1 ELISA KIT ,Bovine CDC2;CDC28A;CDKN1;P34CDC2 ELISA KIT ,Bovine Cyclin-dependent kinase 1 ELISA KIT ,Bovine CDK1 ELISA KIT ,Bovine p34 protein kinase;Cell division control protein 2 homolog;Cyclin-dependent kinase inhibitor 1 ELISA KIT ,Bovine p21 ELISA KIT ,Bovine CDK-interacting protein 1 ELISA KIT ,Bovine Melanoma differentiation-associated protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine cyclin-dependent kinase inhibitor 1,Cyclin-dependent kinase inhibitor 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin-dependent kinase inhibitor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyclin-dependent kinase inhibitor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase inhibitor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyclin-dependent kinase inhibitor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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