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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Vitamin D-binding protein,GC
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Vitamin D-binding protein, GC ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GC,vitamin D binding protein,gc-globulin; DBP,GC; DBP; GRD3; HEL-S-51; VDBG; VDBP; VDB; epididymis secretory protein Li 51; vitamin D-binding alpha-globulin; vitamin D-binding protein; group-specific component, Gc-globulin
Search name
Bovine GC ELISA KIT ,Bovine vitamin D binding protein ELISA KIT ,Bovine gc-globulin ELISA KIT ,Bovine DBP ELISA KIT ,Bovine GC ELISA KIT ,Bovine DBP ELISA KIT ,Bovine GRD3 ELISA KIT ,Bovine HEL-S-51 ELISA KIT ,Bovine VDBG ELISA KIT ,Bovine VDBP ELISA KIT ,Bovine VDB ELISA KIT ,Bovine epididymis secretory protein Li 51 ELISA KIT ,Bovine vitamin D-binding alpha-globulin ELISA KIT ,Bovine vitamin D-binding protein ELISA KIT ,Bovine group-specific component ELISA KIT ,Bovine Gc-globulin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine vitamin d-binding protein,DBP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DBP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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