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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Complement C2,C2
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Complement C2, C2 ELISA KIT
Product Name:Bovine Complement C2, C2 ELISA KIT
Packing:96T
Catalog No.:ELI-05807b
Gene Name:Bovine Complement C2, C2
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Complement C2, C2
Alternative Name:C2,Bovine Complement C2, C2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Complement C2, C2 ELISA KIT allows for the in vitro quantitative determination of Bovine Complement C2, C2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Complement C2, C2 ELISA KIT has been pre-coated with an Bovine Complement C2, C2 antibody specific to Bovine Complement C2, C2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Complement C2, C2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Complement C2, C2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Complement C2, C2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验补体成分 C2(C2a/C2b) 补体成分:C2 血清浓度(μg/ml):20 分子量(kDa):110 亚单位(链)及分子量(kDa):1条 激活产物:C2b;C2a 生物学活性:C2a 丝氨酸蛋白酶,组成CP中的C3、C5转化酶 ELISA KIT & ANTIBODY
Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
. The recombinant hC2 activity is tested in a complement activation ELISA assay that measures classical, alternative, and lectin complement pathway activity in C2-depleted serum.
Determining Alleles of the C2 Gene by Southern Blotting
The second component of human complement, C2, is a 102-kDa single-chain glycoprotein. It provides the catalytic subunit for the C3/C5 convertases of the classical and lectin pathways of complement activation. The single gene encoding C2 spans
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