- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-05791b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Rho-related GTP-binding protein Rho6,RND1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Rho- related GTP- binding protein Rho6, RND1 ELISA KIT
Product Name:Bovine Rho- related GTP- binding protein Rho6, RND1 ELISA KIT
Packing:96T
Catalog No.:ELI-05791b
Gene Name:Bovine Rho-related GTP-binding protein Rho6, RND1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine Rho-related GTP-binding protein Rho6, RND1
Alternative Name:RND1,Bovine Rho-related GTP-binding protein Rho6, RND1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Rho- related GTP- binding protein Rho6, RND1 ELISA KIT allows for the in vitro quantitative determination of Bovine Rho-related GTP-binding protein Rho6, RND1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Rho- related GTP- binding protein Rho6, RND1 ELISA KIT has been pre-coated with an Bovine Rho-related GTP-binding protein Rho6, RND1 antibody specific to Bovine Rho-related GTP-binding protein Rho6, RND1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Rho-related GTP-binding protein Rho6, RND1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Rho-related GTP-binding protein Rho6, RND1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Rho-related GTP-binding protein Rho6, RND1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验小GTP结合蛋白(small GTP binding protein)
), 它催化GDT同GTP的交换。另一种是GTP 酶激活蛋白(GTPase-activating protein, GAP), 它触发结合的GTP水解。 许多小GTP结合蛋白都有一个共价结合的脂基团, 帮助它们同膜结合。小GTP蛋白包括ras、rab和rho蛋白, 它们在一些重要的的活动中起调节作用, 包括分泌活动和细胞骨架的装配等。
Biochemical Assays to Characterize Rho GTPases
of binding on the protein structure and, ultimately, to gain insights into their biological functions. This chapter describes (1) detailed protocols for the expression and purification of Rho GTPases, of �effector-binding domains, and catalytic domains
Activation of Ras and Rho GTPases and MAP Kinases by G-Protein-Coupled Receptors
A complex intracellular signaling network mediates the multiple biological activities of G-protein-coupled receptors (GPCRs). Among them, monomeric GTPases and a family of closely related proline-targeted serine–threonine kinases
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