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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine C-C chemokine receptor type 7,CCR7
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine C- C chemokine receptor type 7, CCR7 ELISA KIT
Product Name:Bovine C- C chemokine receptor type 7, CCR7 ELISA KIT
Packing:96T
Catalog No.:ELI-05400b
Gene Name:Bovine C-C chemokine receptor type 7, CCR7
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine C-C chemokine receptor type 7, CCR7
Alternative Name:CCR7,Bovine C-C chemokine receptor type 7, CCR7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine C- C chemokine receptor type 7, CCR7 ELISA KIT allows for the in vitro quantitative determination of Bovine C-C chemokine receptor type 7, CCR7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine C- C chemokine receptor type 7, CCR7 ELISA KIT has been pre-coated with an Bovine C-C chemokine receptor type 7, CCR7 antibody specific to Bovine C-C chemokine receptor type 7, CCR7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine C-C chemokine receptor type 7, CCR7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine C-C chemokine receptor type 7, CCR7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine C-C chemokine receptor type 7, CCR7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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of ice-cold PBS containing protease inhibitor cocktail. 5. Prepare 0.2X trypsin diluted in PBS, and add 5 mL of it to the cells. Incubate in 5% CO2 for 5 min at 37°C. 6. Neutralize the trypsin with 10 mL of 10% fetal bovine
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
Figures Figure 1.23.1 Saturation binding plot of [3 H]‐mesulergine binding to the 5‐HT2C receptor expressed in CHO
Characterization of Chemokine Receptors
., DiGregorio, D., Mak, J.Y., Horuk, R., and Schall, T.J. 1993. Molecular cloning, functional expression, and signaling characteristics of a C‐C chemokine receptor. Cell. 72:415‐425.
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