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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Aromatic-L-amino-acid decarboxylase,DDC
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Aromatic- L- amino- acid decarboxylase, DDC ELISA KIT
Product Name:Bovine Aromatic- L- amino- acid decarboxylase, DDC ELISA KIT
Packing:96T
Catalog No.:ELI-04672b
Gene Name:Bovine Aromatic-L-amino-acid decarboxylase, DDC
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Aromatic-L-amino-acid decarboxylase, DDC
Alternative Name:DDC,Bovine Aromatic-L-amino-acid decarboxylase, DDC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Aromatic- L- amino- acid decarboxylase, DDC ELISA KIT allows for the in vitro quantitative determination of Bovine Aromatic-L-amino-acid decarboxylase, DDC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Aromatic- L- amino- acid decarboxylase, DDC ELISA KIT has been pre-coated with an Bovine Aromatic-L-amino-acid decarboxylase, DDC antibody specific to Bovine Aromatic-L-amino-acid decarboxylase, DDC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Aromatic-L-amino-acid decarboxylase, DDC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Aromatic-L-amino-acid decarboxylase, DDC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Aromatic-L-amino-acid decarboxylase, DDC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(E2758) BME amino acids solution (B6766) Bovine serum albumin, Fraction V (A3311) Bovine serum albumin, essentially fatty acid free (A6003) Calcium chloride (C7902) Epinephrine (E4250) DL-Lactic acid, sodium salt, 98% syrup (L-
dopamine-β-hydroxylase 多巴胺-β羟化酶 DEAE diethyl-amino-ethyl-dextran 二乙氨乙基葡聚糖 DEPC diethyl pyrocarbonate 焦碳酸二乙酯 DDC dopamine decarboxylase 多巴胺脱羧酶 Des desmine 结蛋白 DIF direct immunofluorescent method 直接免疫荧光法 Dig digoxigenin 地高
Studies of the Ubiquitin Proteasome System
) 10× in vitro translation (IVT) buffer (Promega) Rabbit reticulocyte lysate or wheat germ extract (Promega) Amino acid mixture (Promega) lacking methionine (−Met), cysteine (−Cys) or lysine (−Lys) depending on amino acid
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