Bovine Perilipin- 2, PLIN2 ELI

Bovine Perilipin-2, PLIN2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Bovine PLIN2 ELISA KIT ,Bovine ADFP ELISA KIT ,Bovine ADRP ELISA KIT ,Bovine adipophilin ELISA KIT ,Bovine adipose differentiation-related protein ELISA KIT ,Bovine perilipin 2 ELISA KIT ,Bovine Perilipin-2 ELISA KIT

Intended use

Bovine PLIN2 ELISA KIT allows for the in vitro quantitative determination of bovine Perilipin-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perilipin-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Perilipin-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Perilipin-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Perilipin-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.