- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-04238b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Cathepsin D,CTSD
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Cathepsin D, CTSD ELISA KIT
Product Name:Bovine Cathepsin D, CTSD ELISA KIT
Packing:96T
Catalog No.:ELI-04238b
Gene Name:Bovine Cathepsin D, CTSD
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Cathepsin D, CTSD
Alternative Name:CTSD,Bovine Cathepsin D, CTSD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Cathepsin D, CTSD ELISA KIT allows for the in vitro quantitative determination of Bovine Cathepsin D, CTSD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Cathepsin D, CTSD ELISA KIT has been pre-coated with an Bovine Cathepsin D, CTSD antibody specific to Bovine Cathepsin D, CTSD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Cathepsin D, CTSD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Cathepsin D, CTSD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Cathepsin D, CTSD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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