- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03956b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Calpastatin,CAST
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Calpastatin, CAST ELISA KIT
Product Name:Bovine Calpastatin, CAST ELISA KIT
Packing:96T
Catalog No.:ELI-03956b
Gene Name:Bovine Calpastatin, CAST
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Calpastatin, CAST
Alternative Name:CAST,Bovine Calpastatin, CAST
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Calpastatin, CAST ELISA KIT allows for the in vitro quantitative determination of Bovine Calpastatin, CAST concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Calpastatin, CAST ELISA KIT has been pre-coated with an Bovine Calpastatin, CAST antibody specific to Bovine Calpastatin, CAST .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Calpastatin, CAST and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Calpastatin, CAST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Calpastatin, CAST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Proteins using Small Format 2D Gel Electrophoresis
a standard protein assay kit. Depending on the assay system, the lysis buffer used to prepare the bacterial proteins may give a background equivalent to approximately 5mg/ml of protein as determined from a standard curve; this compares with a protein
Analysis of Proteins using Small Format 2D Gel Electrophoresis
of the sample can be determined using a standard protein assay kit. Depending on the assay system, the lysis buffer used to prepare the bacterial proteins may give a background equivalent to approximately 5mg/ml of protein as determined from a standard curve
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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