- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03833b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Secreted frizzled-related protein 1,SFRP1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Secreted frizzled- related protein 1, SFRP1 ELISA KIT
Product Name:Bovine Secreted frizzled- related protein 1, SFRP1 ELISA KIT
Packing:96T
Catalog No.:ELI-03833b
Gene Name:Bovine Secreted frizzled-related protein 1, SFRP1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Secreted frizzled-related protein 1, SFRP1
Alternative Name:SFRP1,Bovine Secreted frizzled-related protein 1, SFRP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Secreted frizzled- related protein 1, SFRP1 ELISA KIT allows for the in vitro quantitative determination of Bovine Secreted frizzled-related protein 1, SFRP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Secreted frizzled- related protein 1, SFRP1 ELISA KIT has been pre-coated with an Bovine Secreted frizzled-related protein 1, SFRP1 antibody specific to Bovine Secreted frizzled-related protein 1, SFRP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Secreted frizzled-related protein 1, SFRP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Secreted frizzled-related protein 1, SFRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Secreted frizzled-related protein 1, SFRP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
with the Wnt preparation and cellular context. Key words: sFRP , Recombinant expression , Protein purification , Heparin chromatography , Chelating chromatography , Wnt 1. Introduction Secreted Frizzled-related proteins (sFRPs) contain a Frizzled(Fzd
Analysis of Oxidative Modification of Proteins
6.8 ( appendix 2A ) Bicinchoninic acid protein assay kit (BCA; Pierce Co.) Bovine serum albumin (BSA
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
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