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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Mucin-15,MUC15
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Mucin-15, MUC15 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Mucin-15 MUC15,MUC-15; PAS3; PASIII; mucin 15, mucin 15cell surface associated
Search name
Bovine Mucin-15 MUC15 ELISA KIT ,Bovine MUC-15 ELISA KIT ,Bovine PAS3 ELISA KIT ,Bovine PASIII ELISA KIT ,Bovine mucin 15 ELISA KIT ,Bovine mucin 15cell surface associated ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine mucin-15,MUC-15 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUC-15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MUC-15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MUC-15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MUC-15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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