- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03349b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Vimentin,VIM
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Vimentin, VIM ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
VIM,Vimentin,CTRCT30; HEL113; epididymis luminal protein 113
Search name
Bovine VIM ELISA KIT ,Bovine Vimentin ELISA KIT ,Bovine CTRCT30 ELISA KIT; Bovine HEL113 ELISA KIT; Bovine epididymis luminal protein 113 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Vimentin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vimentin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Vimentin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Vimentin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vimentin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma
electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry, our groups have identified lamin B1 (LMNB1) and vimentin (VIM) as promising biomarkers for detection of early HCC. Protein levels of both biomarkers were significantly elevated in cancerous tissues
所发生的肿瘤，是肿瘤病理学中最棘手和最疑难的问题之一。随着免疫组化技术在肿瘤鉴别诊断中的应用，已经克服了许多以往无法克服或者难以克服的问题。当前，应用于这方面检测的抗体越来越多，下面将介绍几种常用的抗体： 1.波形蛋白(Vimentin ,Vim) Vim是原始的中间丝，它存在于所有的间叶组织细胞中，可以向神经胶质，肌源性细胞和上皮细胞中间丝演变。Vim是一种单克隆抗体，临床应用来作鉴别诊断的有：鉴别癌与内癌，前者阴性，后者阳性;鉴别小细胞性肿瘤，这时应多用几种抗体，如CK
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
of the quality and quantity of remodeling of epithelial cells is a complex issue. It usually includes quantification of expression of epithelial and mesenchymal markers (E-cadherin, N-cadherin, and vimentin), visualization of cytoskeletal rebuilding (F-
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