- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03266b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Alpha lactalbumin,LALBA
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Alpha lactalbumin, LALBA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Alpha-lactalbumin,Lactose synthase B protein, LALBA,lactose synthase B protein; alpha-lactalbumin; lysozyme-like protein 7; lactalbumin alpha
Search name
Bovine Alpha-lactalbumin ELISA KIT ,Bovine Lactose synthase B protein ELISA KIT ,Bovine LALBA ELISA KIT ,Bovine lactose synthase B protein ELISA KIT ,Bovine alpha-lactalbumin ELISA KIT ,Bovine lysozyme-like protein 7 ELISA KIT ,Bovine lactalbumin alpha ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine alpha-lactalbumin,Alpha-lactalbumin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-lactalbumin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-lactalbumin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-lactalbumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-lactalbumin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验而言，他们利用悬浮球培养使得 iPSCs 分化成拟胚体（embryoid bodies，mEBs）。随后，研究人员将 mEBs 培养于 3D 培养系统，生成类似于乳腺的类器官。这一培育过程需要用到基质胶、乳腺发育相关的激素及生长因子等化合物。二步法构建乳腺类器官的过程[3]基于二步法获得的乳腺类器官，经验证成功表达有乳腺标志物（例如 lactalbumin/LALBA、EpCAM、 CK14 和 P63）。这意味着，在特定条件下由 iPSCs 分化、培育而来的类器官具备乳腺的关键特征。虽然尚且不能完美复制
(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse
Endothelial Cell Systems - Instructions For Use
recombinant Epidermal Growth Factor) 1µg/ml Hydrocortisone 50 µg/ml Gentamicin, 50 ng/ml Amphotericin-B 3 mg/ml BBE (Bovine Brain Extract) (CC-4092), 2 ml 2% FBS (Fetal Bovine Serum) CC-3124 EGM® BulletKit ® Kit which contains a 500 ml bottle
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