- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-03103b
- 2025年07月15日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Poly [ADP-ribose] polymerase 1,PARP1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Poly [ADP- ribose] polymerase 1, PARP1 ELISA KIT
Product Name:Bovine Poly [ADP- ribose] polymerase 1, PARP1 ELISA KIT
Packing:96T
Catalog No.:ELI-03103b
Gene Name:Bovine Poly [ADP-ribose] polymerase 1, PARP1
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Poly [ADP-ribose] polymerase 1, PARP1
Alternative Name:PARP1,Bovine Poly [ADP-ribose] polymerase 1, PARP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Poly [ADP- ribose] polymerase 1, PARP1 ELISA KIT allows for the in vitro quantitative determination of Bovine Poly [ADP-ribose] polymerase 1, PARP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Poly [ADP- ribose] polymerase 1, PARP1 ELISA KIT has been pre-coated with an Bovine Poly [ADP-ribose] polymerase 1, PARP1 antibody specific to Bovine Poly [ADP-ribose] polymerase 1, PARP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Poly [ADP-ribose] polymerase 1, PARP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Poly [ADP-ribose] polymerase 1, PARP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Poly [ADP-ribose] polymerase 1, PARP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验PARP1 Genomics: Chromatin Immunoprecipitation Approach Using Anti-PARP1 Antibody (ChIP and ChIP-seq)
Poly(ADP-ribose) polymerase1 (PARP1) is a global regulator of different cellular mechanisms, ranging from DNA damage repair to control of gene expression. Since PARP1 protein and pADPr have been shown to persist in chromatin through cell
Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors
During the last few years, poly(ADP-ribose)polymerase (PARP) proteins became a very popular target for anticancer treatment. Many PARP inhibitors have been generated and tested by pharmacological industry. However, most of them were designed
-3及对底物多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase，PARP]等的裂解。 方法： 收集细胞→PBS洗涤→抽提细胞裂解液→蛋白定量→SDS-PAGE电泳→硝酸纤维素膜或PVDF膜转移→5%脱脂奶粉封闭，室温1.5~2 h或4 °C过夜→Caspase-3多抗或单抗室温反应1~2 h或4 °C过夜→TBS-T(含0.05% Tween 20的TBS)洗3次，5~10 min/次→HRP-标记的羊抗鼠IgG或AP标记的羊抗鼠IgG室温反应1~2h→ TBS
技术资料暂无技术资料 索取技术资料
