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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Heat shock protein beta-6,HSPB6
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Heat shock protein beta- 6, HSPB6 ELISA KIT
Product Name:Bovine Heat shock protein beta- 6, HSPB6 ELISA KIT
Packing:96T
Catalog No.:ELI-02938b
Gene Name:Bovine Heat shock protein beta-6, HSPB6
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Heat shock protein beta-6, HSPB6
Alternative Name:HSPB6,Bovine Heat shock protein beta-6, HSPB6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Heat shock protein beta- 6, HSPB6 ELISA KIT allows for the in vitro quantitative determination of Bovine Heat shock protein beta-6, HSPB6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Heat shock protein beta- 6, HSPB6 ELISA KIT has been pre-coated with an Bovine Heat shock protein beta-6, HSPB6 antibody specific to Bovine Heat shock protein beta-6, HSPB6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Heat shock protein beta-6, HSPB6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Heat shock protein beta-6, HSPB6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Heat shock protein beta-6, HSPB6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Procedures for In Vitro Production of Bovine Embryos
, Chenoweth PJ, Hansen PJ. Deleterious actions of gossypol on bovine spermatozoa, oocytes and embryos. Biol Reprod 1997; 57, 901-907.Edwards JL, Ealy AD, Monterroso VH, Hansen PJ. Ontogeny of temperature-regulated heat shock protein 70 synthesis
Strategies to Optimize Protein Expression in E. coli
. Biol. Chem. 277:50564‐50572. Phillips, T.A., Vanbogelen, R.A., and Neidhardt, F.C. 1984. Ion gene‐product of Escherichia coli is a heat‐shock protein. J. Bacteriol. 159:283
synthase kinase 3beta as a target for the therapy of shock and inflammation. Shock. 27 , 113–123. 8. Rubinfeld B, Albert I, Porfiri E, Fiol C, Munemitsu S, Polakis P. (1996) Binding of GSK3beta to the APC-beta-catenin complex
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