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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Glycogen phosphorylase,muscle form,PYGM
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT
Product Name:Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT
Packing:96T
Catalog No.:ELI-02890b
Gene Name:Bovine Glycogen phosphorylase, muscle form, PYGM
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Glycogen phosphorylase, muscle form, PYGM
Alternative Name:PYGM,Bovine Glycogen phosphorylase, muscle form, PYGM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT allows for the in vitro quantitative determination of Bovine Glycogen phosphorylase, muscle form, PYGM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT has been pre-coated with an Bovine Glycogen phosphorylase, muscle form, PYGM antibody specific to Bovine Glycogen phosphorylase, muscle form, PYGM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Glycogen phosphorylase, muscle form, PYGM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Glycogen phosphorylase, muscle form, PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Glycogen phosphorylase, muscle form, PYGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mol Cell:黄波团队揭示糖原代谢调控记忆性 T 细胞早期应答反应
途径产生 NADPH,NADPH 维持细胞内氧化还原稳态,保证 Tm 细胞二次激活反应时的高质量和高活性。 糖原磷酸化酶(Glycogen phosphorylase,PYG)是糖原分解反应的关键酶,催化糖原裂解为 1 磷酸葡萄糖,具有三种亚型(PYGL,Liver;PYGM, Muscle;PYGB, Brain)。本研究发现 PYGB 在 T 细胞中高表达。 重要的是,PYG 一直被认为通过其丝氨酸磷酸化而发挥活性功能;本研究还首次发现 TCR 的下游信号分子 ZAP70/LCK 能够
PriCells: Introduction of Isolation and Culture of Human Primary Hepatocytes
, the synthesis of cholesterol and bile acids, and the metabolism of drugs, steroids, and amino acids. In addition, the liver has a central role in energy metabolism as the major store of glycogen and the site of gluconeogenesis and synthesis of fatty acids
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
., and Cohen, P. (1980) Glycogen synthase kinase-3 from rabbit skeletal muscle. Separation from cyclic-AMP-dependent protein kinase and phosphorylase kinase. Eur. J. Biochem. 107, 519–527. 6. Cohen, P. and Goedert, M. (2004) GSK3 inhibitors: development
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