- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-02642b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Hexokinase-1,HK1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Hexokinase- 1, HK1 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Hexokinase-1,Brain form hexokinase,Hexokinase type I,HK I,HK1,hexokinase-1, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, HK
I, brain form hexokinase, glycolytic enzyme, hexokinase type I, hexokinase 1
Search name
Bovine Hexokinase-1 ELISA Kit, Bovine HK1 ELISA Kit, Bovine Brain form hexokinase ELISA KIT,Bovine Hexokinase type I
ELISA KIT,Bovine HK I ELISA KIT,Bovine HK1 ELISA KIT,Bovine hexokinase-1 ELISA KIT,Bovine HK1-ta ELISA KIT,Bovine
HK1-tb ELISA KIT,Bovine HK1-tc ELISA KIT,Bovine HKD ELISA KIT,HumanHKI ELISA KIT,Bovine HMSNR ELISA KIT,Bovine
HXK1 ELISA KIT,Bovine HK I ELISA KIT,Bovine brain form hexokinase ELISA KIT,Bovine glycolytic enzyme ELISA KIT,Bovine
hexokinase type I ELISA KIT,Bovine hexokinase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Hexokinase-1 concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Hexokinase-1, During the reaction, Hexokinase-1 in the sample or standard competes with a fixed
amount of biotin-labeled Hexokinase-1 for sites on a pre-coated Monoclonal antibody specific to Hexokinase-1. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Hexokinase-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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