- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-02415b
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine heat shock protein 27,HSP27
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine heat shock protein 27, HSP27 ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Heat shock protein beta-1(HspB1),Heat shock 27 kDa protein(HSP 27),; HSP 27; CMT2F; HMN2B; HS.76067; HSP28; Hsp25; SRP27;
28 kDa heat shock protein; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock protein
beta-1; stress-responsive protein 27; heat shock 27kDa protein 1,
Search name
Bovine Heat shock protein beta-1 ELISA Kit, Bovine HspB1 ELISA Kit, Bovine Heat shock 27 kDa protein ELISA Kit, Bovine HSP
27 ELISA Kit, Bovine CMT2F ELISA Kit, Bovine HMN2B ELISA Kit, Bovine HS.76067 ELISA Kit, Bovine HSP28 ELISA Kit, Bovine
Hsp25 ELISA Kit, Bovine SRP27 ELISA Kit, Bovine stress-responsive protein 27 ELISA Kit, Bovine Heat shock 28 kDa protein
ELISA Kit, Bovine Heat shock 25 kDa protein ELISA Kit, Bovine estrogen-regulated 24 kDa protein ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine heat shock protein beta-1,HspB1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HspB1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HspB1 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain HspB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HspB1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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文献和实验StructureFunctions of HspB1 (Hsp27)
Human HspB1 (also denoted Hsp27) is a well-known member, together with alphaB-crystallin, of the small heat-shock (or stress) proteins (sHsps) (20–40 kDa). In this chapter, I describe procedures for testing the oligomeric and phosphorylation
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
Quantification of HSP27 and HSP70 Molecular Chaperone Activities
Stress-inducible heat-shock proteins (HSPs, like HSP70 and HSP27) are molecular chaperones that �protect cells from stress damage by keeping cellular proteins in a folding competent state and preventing them from irreversible aggregation. HSP27
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