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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Pulmonary surfactant-associated protein C,SP-C ELISA K
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Pulmonary surfactant- associated protein C, SP- C ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Pulmonary surfactant-associated protein C(SP-C),Pulmonary surfactant-associated proteolipid SPL(Val),
Search name
Bovine Pulmonary surfactant-associated protein C ELISA Kit, Bovine SP-C ELISA Kit,Pulmonary surfactant-associated protein
C ELISA Kit,SP-C ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine pulmonary surfactant-associated protein c,SP-C
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-C. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SP-C and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain SP-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SP-C in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
PDF
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