- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0543b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Immunoglobulin M
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Immunoglobulin M ELISA Kit
Product Name:Bovine Immunoglobulin M ELISA Kit
Packing:96T
Catalog No.:ELA-E0543b
Gene Name:Bovine Immunoglobulin M
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Bovine Immunoglobulin M
Alternative Name:IgM,Bovine Immunoglobulin M
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Immunoglobulin M ELISA Kit allows for the in vitro quantitative determination of Bovine Immunoglobulin M concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Immunoglobulin M ELISA Kit has been pre-coated with an Bovine Immunoglobulin M antibody specific to Bovine Immunoglobulin M .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Immunoglobulin M and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Immunoglobulin M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Immunoglobulin M in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验TUNEL PROCEDURE IN BOVINE EMBRYOS
away whatever is not used in one day. 4% (v/v) paraformaldehyde: 1:1 solution of 8% paraformaldehyde stock solution and 0.2 M PBS. Make up on the day of use. Microscope slides: dip the slides in
TUNEL PROCEDURE IN BOVINE EMBRYOS
/v) paraformaldehyde: 1:1 solution of 8% paraformaldehyde stock solution and 0.2 M PBS. Make up on the day of use. Microscope slides: dip the slides in 1:10 poly-l-lysine solution (Sigma P8920) for 2 minutes. Allow the slides
80150ml of PBS , 0.01 M, PH 7.4Reference/More informationVoller A , Bartlett A, Bidwell DE, Clark MF, Adams An, 1976. The detection of viruses by enzyme-linked immunosorbent assay (ELISA). J Gen Virol 33, 165-167.Figure 1
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