- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0526b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Platelet-activating factor,PAF
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Platelet- activating factor, PAF ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Platelet-activating factor,; PAFA,PAF, PAF-acether , AGEPC,acetyl-glyceryl-ether-phosphorylcholine
Search name
Bovine Platelet- activating factor ELISA Kit, Bovine PAF ELISA Kit, Bovine PAFA ELISA Kit, Bovine PAF-acether ELISA Kit,
Bovine AGEPC ELISA Kit, Bovine acetyl-glyceryl-ether-phosphorylcholine ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of platelet-activating factor,Platelet-activating factor
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Platelet-activating factor, During the reaction, Platelet-activating factor in the sample or standard
competes with a fixed amount of biotin-labeled Platelet-activating factor for sites on a pre-coated Monoclonal antibody specific to
Platelet-activating factor. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Platelet-activating factor in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Human Plasma Platelet-Activating Factor Acetylhydrolase
The human plasma platelet-activating factor acetylhydrolase (PAF acetylhydrolase, l-alkyl-2-acetyl-glycerophosphocholine esterase, l-alkyl-2-acetyl- sn -glycero-3-phosphocholine acetohydrolase, EC 3.1.1.47) is a phospholipase A 2 activity
Modified Microchemotaxis Assays
leukocyte migration, including activated serum components, platelet activating factor (PAF), eucosinoids, cytokines (e.g., IL-1, TNFα, IFN-γ), and neuroendocrine hormones (1 ). Although many of these factors are believed to play some role in inflammation
一、趋化因子 由组织细胞和微生物产生的趋化剂（chemoattractants)对白细胞的趋化作用（chemotaxis)是炎症发生过程中重要的起始步骤，也是机体防御和清除入侵病原体等异物先天性免疫功能的一个重要方面。1986年以前，“经典”的（classical)白细胞趋化物质主要有补体片段C5a、白三烯B4（leukotrinin B4,LTB4)、血小板激活因子（platelet-activating factor,PAF
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