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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine endotoxin,ET
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Endotoxin ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Endotoxin,;
Search name
Bovine Endotoxin ELISA Kit, Bovine ET ELISA Kit,Endotoxin ELISA Kit,ET ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of endotoxin,Endotoxin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Endotoxin, During the reaction, Endotoxin in the sample or standard competes with a fixed amount
of biotin-labeled Endotoxin for sites on a pre-coated Monoclonal antibody specific to Endotoxin. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Endotoxin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验犬 内毒素 (endotoxin) 酶联免疫分析(ELISA) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定犬血清,血浆及相关液体样本中 内毒素 (ET) 的 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 犬 内毒素 (ET) 水平。 用纯化的 犬 内毒素 (ET) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 内毒素 (ET) 再与HRP标记的ET抗体结合,形成抗体
Procedures for In Vitro Production of Bovine Embryos
Procedures for In Vitro Production of Bovine EmbryosThe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)] of embryos described here are based on procedures developed
R&D Systems Quantikine® 人高分子量脂联素ELISA试剂盒
浆中HMW 脂联素的水平,而不需要对样品进行蛋白水解的预处理。关于脂联素相关产品的更多信息,请访问我们的网站www.RnDSystems.com/Adipocytokines。人HMW 脂联素水平的测定。利用Quantikine Human HMW Adiponectin/Acrp30ELISA Kit(货号 DHWAD0),我们分析了来自不同BMI 个体的血清中的HMW 脂联素平均水平,包括低BMI(平均BMI = 19.8 ;N=4),高BMI(平均BMI = 32.8 ;N=4)和超高BMI(平均
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