AF1Q (Protein AF1q) ELISA Kit/

Mouse AF1Q (Protein AF1q) ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse MLLT11 ELISA KIT ,Mouse TUJ1 ELISA KIT ,Mouse AF1Q ELISA KIT ,Mouse ALL1 fused gene from chromosome 1q ELISA KIT ,Mouse ALL1-fused gene from chromosome 1q ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog Drosophila) ELISA KIT ,Mouse translocated to 11 ELISA KIT ,Mouse myeloid/lymphoid or mixed-lineage leukemia ELISA KIT ,Mouse translocated to 11 ELISA KIT

Intended use

Mouse AF1Q ELISA kit allows for the in vitro quantitative determination of Mouse Protein AF1q concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse AF1Q ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AF1Q, During the reaction, AF1Q in the sample or standard competes with a fixed amount of biotin-labeled AF1Q for sites on a pre-coated Monoclonal antibody specific to AF1Q. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AF1Q in the samples is then determined by comparing the O.D. of the samples to the standard curve.