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- 详细信息
- 文献和实验
- 技术资料
- 灵敏度:
46.9pg/ml
- 库存:
50
- 供应商:
LSM Bio
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测 小鼠样本GSDMDC1蛋白浓度
- 适应物种:
小鼠
- 标记物:
GSDMDC1
- 样本:
小鼠血清,血浆,组织匀浆
- 规格:
96T
Mouse GSDMDC1 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse GSDMDC1C1 ELISA KIT ,Mouse DF5L ELISA KIT ,Mouse DFNA5L ELISA KIT ,Mouse FKSG10 ELISA KIT ,Mouse GSDMDC1C1C1 ELISA KIT ,Mouse gasdermin domain containing 1 ELISA KIT ,Mouse gasdermin domain-containing protein 1 ELISA KIT ,Mouse gasdermin-D ELISA KIT ,Mouse gasdermin D ELISA KIT
Intended use
Mouse GSDMDC1 ELISA kit allows for the in vitro quantitative determination of Mouse GSDMDC1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse GSDMDC1 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GSDMDC1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GSDMDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GSDMDC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Experimental and Therapeutic Medicine
Published online on: April 15, 2020
doi.org/10.3892/etm.2020.8667
Pages: 261-268
Article: Role of recombinant human brain natriuretic peptide combined with sodium nitroprusside in improving quality of life and cardiac function in patients with acute heart failure
Authors: Yang Peng Han Wei
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs
A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates. The ELISA method allows for a much faster quantitation of virus in infected organs than plaque
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