Mouse GPHN (Gephyrin) ELISA Ki

Mouse GPHN (Gephyrin) ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse GPHN ELISA KIT ,Mouse gephyrin ELISA KIT ,Mouse GEPH ELISA KIT ,Mouse GPH ELISA KIT ,Mouse GPHRYN ELISA KIT ,Mouse HKPX1 ELISA KIT

Intended use

Mouse GPHN ELISA kit allows for the in vitro quantitative determination of Mouse Gephyrin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse GPHN ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPHN, During the reaction, GPHN in the sample or standard competes with a fixed amount of biotin-labeled GPHN for sites on a pre-coated Monoclonal antibody specific to GPHN. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GPHN in the samples is then determined by comparing the O.D. of the samples to the standard curve.