Human RNA- binding protein FUS

Human RNA-binding protein FUS, FUS ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests Operating instruction

Alternative name

Human FUS ELISA KIT ,Human FUS RNA binding protein ELISA KIT ,Human oncogene FUS ELISA KIT ,Human fus-like protein ELISA KIT ,Human ALS6 ELISA KIT ,Human ETM4 ELISA KIT ,Human FUS1 ELISA KIT ,Human HNR2 ELISA KIT ,Human POMP75 ELISA KIT ,Human TLS ELISA KIT ,Human 75 kDa DNA-pairing protein ELISA KIT ,Human fused in sarcoma ELISA KIT ,Human fusion gene in myxoid liposarcoma ELISA KIT ,Human heterogeneous nuclear ribonucleoprotein P2 ELISA KIT ,Human oncogene TLS ELISA KIT ,Human translocated in liposarcoma protei ELISA KIT ,Human RNA-binding protein FUS ELISA KIT

Intended use

Human FUS ELISA KIT allows for the in vitro quantitative determination of human RNA-binding protein FUS concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human FUS ELISA KIT has been pre-coated with an antibody specific to RNA-binding protein FUS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forRNA-binding protein FUS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RNA-binding protein FUS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RNA-binding protein FUS in the samples is then determined by comparing the O.D. of the samples to the standard curve.