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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
secreted protein, acidic, cysteine-rich (osteonectin)
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse ,Rat,Pig
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
P67936
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
secreted protein, acidic, cysteine-rich (osteonectin)
- 抗体英文名:
anti-SPARC antibody,SPARC antibody
- 抗体名:
anti-SPARC 抗体,SPARC 抗体
- 规格:
100μg
SPARC抗体| SPARC antibody
货号 PAab08153
蛋白别名 ON
蛋白介绍
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
产品描述
anti-SPARC antibody is a Rabbit Polyclonal antibody againstSPARC..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human stomach cancer using PAab08153(SPARC antibody) at dilution of 1:100
Western blot
human placenta tissue were subjected to SDS PAGE followed by western blot with PAab08153(SPARC antibody) at dilution of 1:500(本抗体仅供体外科研用途,不可用于临床诊断!) "">
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文献和实验Circulation:华科协和医院程翔等报道组织特异性Treg 为基础的心脏免疫治疗新思路
的机制 为进一步阐明心肌梗死后心脏 Tregs 促进组织修复的机制,研究人员对心脏 Tregs 的转录组数据进行分析,发现 Sparc 是心脏高表达差异基因中最显著的差异基因之一,实时定量 PCR 结果也证实了心脏 Sparc 的高转录水平。作为一种胶原结合母细胞蛋白,Sparc 在胶原组装成细胞外基质 (ECM) 中起关键作用,已有报道其在心肌梗死后的心室完整性保护中起关键作用。 图片来源:Circulation为了从功能上确定 Sparc 是否在心肌梗死后心脏 Tregs 介导的组织修复中
Protein detection onto PVDF membranes
[3]. Scanning The Laser Densitometer (4000 x 5000 pixels; 12 bits/pixel) from Molecular Dynamics and the GS-700 from Bio-Rad have been used as scanning device. This scanners were linked to Sparc workstations and Macintosh computers.
(2)第一抗体封闭: SPARC单抗以T-TBS 1:200稀释,按0.1ml/cm2加入封有硝酸纤维素膜的杂交袋中,去除所有气泡,4℃振摇过夜。 ------------------------------------------------------------------------------------------------------- (3)以T-TBS洗膜,10分钟´ 3次。 (4)第二抗体封闭:辣根过氧化物酶(HRP)标记
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