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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9GZT9
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
CLP1
- 抗体英文名:
anti-CLP1 antibody,CLP1 antibody
- 抗体名:
anti-CLP1 抗体,CLP1 抗体
- 规格:
100μg
CLP1抗体| CLP1 antibody
货号 PAab01770
蛋白别名 HEAB
蛋白介绍
Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
产品描述
anti-CLP1 antibody is a Rabbit Polyclonal antibody againstCLP1..
建议稀释比例
IHC
Western blot
(本抗体仅供体外科研用途,不可用于临床诊断!)
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文献和实验在免疫反应方面,基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体,按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等,但这些并不等于有相应的不同抗体存在,一般多只表明抗体反应的多样性而已。抗体在血清中含量最多(体液抗体=humoral antibody);含有某特定抗体的血清称为抗血或免疫血清。
机体在抗原物质刺激下,由B细胞分化成的浆细胞所产生的、可与相应抗原发生特异性结合反应的免疫球蛋白。因为最初有人用电泳证明血清中抗体活性在γ球蛋白部分,故曾把抗体统称为两种(γ)球蛋白。后来证明,抗体并不都在γ区;而且位于γ区的球蛋白,也不一定都具有抗体活性。1964年,世界卫生组织举行专门会议,将具有抗体活性以及与抗体相关的球蛋白统称为免疫球蛋白(Ig)。如骨髓瘤蛋白,巨球蛋白血症、冷球蛋白血症等患者血清中存在的异常免疫球蛋白以及“正常人”天然存在的免疫
施一公团队再获新进展!解析人类 pre-tRNA 剪接机制,完善剪接体结构「地图」
步骤产生,从 pre-tRNA 中去除内含子对于生物遗传进化是必不可少的。 在人类中,去除 pre-tRNA 中内含子的过程是由 tRNA 剪接内切核酸酶(TSEN)介导的,该亚酶包括四个亚基:TSEN2、TSEN15、TSEN34 和 TSEN54。值得一提的是,尽管多核苷酸激酶 CLP1 在体外对前 tRNA 切割是可有可无的,但 CLP1 中的突变和所有 TSEN 亚基都与 tRNA 代谢和神经病理学的改变有关。 为回答 pre-tRNA 是如何被 TSEN 识别的,以及 5' 剪接
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