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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
protein kinase, AMP-activated, gamma 1 non-catalytic subunit
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse ,Rat
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9GZT9
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
AMPK gamma 1
- 抗体英文名:
anti-AMPK gamma 1 antibody,AMPK gamma 1 antibody
- 抗体名:
anti-AMPK gamma 1 抗体,AMPK gamma 1 抗体
- 规格:
100μg
AMPK gamma 1抗体| AMPK gamma 1 antibody
货号 PAab00379
蛋白别名 AMPK gamma1 antibody, AMPK subunit gamma 1 antibody, AMPKG antibody, PRKAG1
蛋白介绍
Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and -hydroxy-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK.
产品描述
anti-AMPK gamma 1 antibody is a Rabbit Polyclonal antibody againstAMPK gamma 1..
建议稀释比例
WB :1:500-1:5000;IHC:1:20-1:200
IHC
Immunohistochemistry of paraffin-embedded human testis tissue slide using PAab00379( PRKAG1 Antibody) at dilution of 1:50
Western blot
K-562 cells were subjected to SDS PAGE followed by western blot with PAab00379( PRKAG1 Antibody) at dilution of 1:1000(本抗体仅供体外科研用途,不可用于临床诊断!)
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
起来以检测蛋白的数量;阳性和阴性对照也需要使用在试剂盒中GTP和 GTP-gamma-S reagents进行准备.然后将PAK-1 PBD beads加入到细胞裂解液中(10 ul of beads for every 500 ul of cell lysate).接着孵育以保证Rac1-GTP的结合,使用buffer 清洗the beads以去除细胞碎片和没有结合的Rac1。 最后,加入 laemmli buffer (not provided)到 beads,将泥浆使用anti-Rac
决定簇(antigenic determinant),或称为表位(epitope)。一个抗原分子可带有不同的决定簇。二、抗体(一)抗体的结构抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig分五类,即IgG、IgA、IgM、IgD和IgE.与免疫测定有关的Ig主要为IgG和IgM.Ig由两个轻链(L)和两个重链(H)的单体组成。Ig的轻链是相同的,有κ(kappa)和λ(Lambda)两种型别。五类Ig的重链结构不同,这决定了它们的抗原性也不同。IgG和IgM的重链分别称为γ(gamma
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