- 询价
- GeneTex
- 美国
- GTX35106
- 2026年02月03日
- ICC/IF, IHC-P, FACS
- Ms
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 目录编号：
GTX35106
- 库存：
50
- 供应商：
广州市左克生物
- 宿主：
Ms
- 应用范围：
ICC/IF, IHC-P, FACS
- 抗体英文名：
TGF alpha antibody [P/T1]
- 规格：
25ul/100ul
应用范围：ICC/IF, IHC-P, FACS
适用物种：Hu,Zfsh,Rb
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文献和实验LABELING ALPHA-ACTININ WITH IATR
of injection buffer)and reading OD555. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 100,000} 13.Dilute to 5 mg/ml for microinjection if necessary.Extra conjugated alpha-actinin can be stored as aliquots in liquid N2.
ALPHA FACTOR ARREST AND RELEASE
of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8 M). To release cells from alpha factor arrest, spin down cells and wash twice (using a large volume, dependent on the size of the cell pellet anticipated
In situ hybridisation to alpha satellite sequences (chromosome specific)
anti-mouse HRP antibody diluted 1:80 for 40 minutes at 37C We use DAKO P0260 (12) Wash with blocking solution for 10 minutes (13) Incubate with swine anti-rabbit HRP antibody diluted 1:100 for 40 minutes at 37C We use DAKO P0217 (14
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