- 询价
- GeneTex
- 美国
- GTX60351
- 2026年01月31日
- WB, ICC/IF, Dot, ELISA, ChIP assay
- Rb
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 目录编号：
GTX60351
- 库存：
50
- 供应商：
广州市左克生物
- 宿主：
Rb
- 应用范围：
WB, ICC/IF, Dot, ELISA, ChIP assay
- 抗体英文名：
Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade
- 规格：
25ul/100ul
应用范围：WB, ICC/IF, Dot, ELISA, ChIP assay
适用物种：Hu,Zfsh
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文献和实验Chromatin Immunoprecipitation (ChIP)
of formaldehyde in the ChIP method was pioneered by Solomon and Varshavsky (Solomon et al. 1988). In their original studies, the association of histone H4 and RNA polymerase II with the Drosophila hsp70 genes was monitored. Cells were treated with formaldehyde
. However, to address in detail what happens at specific sites in vivo, chromatin immunoprecipitation (ChIP) is the method of choice. Here, we describe how ChIP can be performed on non-fixed chromatin from animal cells or tissues (fresh or frozen) to analyze histone
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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