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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
of the microscopic field is covered with organelles.For setting up the membrane MT motility assay,you will make need a stock that is 6 x the dilution just determined,which will be called 6X MEMBRANES. *=As published in... Waterman-Storer,C.M.(In press)Microtubule
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Table I Species Protein A Protein G Human ++++ ++++ Mouse ++ ++ Rat
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