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文献和实验Oxidized LDL and Lp(a): Preparation, Modification, and Analysis
During the last several years, it has become increasingly apparent that oxidation of low density lipoproteins (LDL) takes place in vivo as a key event in the development of human atherosclerosis (1 ,2 ) and vascular dysfunction
Purification of Human Complement Protein C5
Complement C5 is cleaved by proteolysis in the terminal phase of complement activation generating the pro-inflammatory C5a and membrane attack complex nucleator C5b. Whereas purification of its paralogues C3 and C4 from plasma is relatively
Analysis of Protein Phosphorylation in Human Neutrophils
, serines and threonines. Upon covalent binding of phosphates to these amino acids, the charge and conformation of the corresponding proteins are modified, generally leading to changes in protein functions such as protein-protein interactions and enzymatic
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