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文献和实验Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies
bodies and optimal conditions have to be determined empirically for any given protein. Here, we describe a simple and efficient refolding protocol for the catalytic domain of type 4 cyclic nucleotide phosphodiesterases (PDE4s). This method
Computational Prediction of Domain Interactions
and carefully curated for protein–protein interactions, as of today little useful data is available from major databases with respect to relations on the domain level. This lack of data makes computational prediction of domain–domain interactions
Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
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