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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Purification of Antiserum or Ascites by Protein A/G Chromatography
of AntibodyAgaroseRabbit IgGProtein A or Protein GMouse IgG1Protein GMouse IgG2Protein A or Protein GMouse IgG3Protein GSheep IgGProtein G but binds weaklyRat IgGProtein G but binds weaklyGuinea Pig IgGProtein ADog IgGProtein AGoat IgGProtein GPig IgGProtein A(2) Buffer
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
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