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文献和实验Receptor Isolation and Characterization: From Protein to Gene
microsequencing. Upon completion of the human genome project, a partial protein sequence is enough for retrieving its complete mRNA and hence its complete protein sequence.
1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
Regulation of the Transcription of G Protein-Coupled Receptor Genes
G protein-coupled receptors (GPCRs) participate in a variety of physiological functions and are major targets of pharmaceutical drugs. More than 600 GPCRs have been identified in the human genome. Although GPCRs are expressed
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