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文献和实验1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA
Phospholipid hydroperoxide glutathione peroxidase (PHGPX or GPX4, E.C. 1.11.1.12) is one of the four identified selenium-dependent glutathione peroxidases (GPX) in mammals (1 ). Both of the pig (2.8 kb) and the mouse (4.0 kb) GPX4 genes
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
of G protein-coupled receptors, vomeronasal receptor type 2 (V2Rs), expressed in the pheromone-detecting vomeronasal organ (VNO) (Chamero et al., Nature 450:899–902, 2007; Haga et al., Nature 466:118–122, 2010; Kimoto et al., Curr Biol 17:1879–1884
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