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文献和实验Immortalised cultured cells are powerful tools to assess the function of ectopically expressed proteins. However, it must be ensured that the protein of interest is functional in the host system and display native behaviour. In particular
Expressing Full-Length Functional PfEMP1 Proteins in the HEK293 Expression System
weight, which further complicates expression of these proteins in heterologous systems. The high molecular weight Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) adhesins expressed on the surface of the infected erythrocytes
6. Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) 实验设备 5 mL Culture Tubes 实验步骤 1. Remove liver aseptically. 2. Flash-freeze liver in foil packets using liquid nitrogen. Store
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