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文献和实验An increased level of cytosolic free calcium (Ca2+ ) is an essential second messenger for apical organelle discharge in Plasmodium falciparum merozoites. Here, we describe a method for isolation of viable and invasive P. falciparum merozoites
of DNA and RNA in solutions of high Cs+ concentration, makes it possible to extract and retrieve simultaneously both DNA and RNA, thus making maximum use of precious parasite mate- rial. This chapter details such a method adapted for P falciparum
Overview of Protein Microarrays
.A., Blair, P.L., Aguiar, J.C., Baldi, P., Davies, D.H., and Felgner, P.L. 2008. Profiling humoral immune responses to P. falciparum infection with protein microarrays. Proteomics 8:4680‐4694.
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