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文献和实验regulated. We have shown that heterotrimeric G proteins regulate assembly and maintenance of epithelial cell barrier in cultured Madin–Darby canine kidney (MDCK) cells. The inducible expression of mutant signaling molecules (constitutively active or dominant
Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay
of host immune responses to the vector. Recent studies have demonstrated cellular immune responses specific to capsid proteins of various AAV serotypes in animal models and in human trials for different diseases. We developed a canine-specific ELISPOT
Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway
, whereas the vacuoles, like RER and Golgi, remain in the cell body. Thus, polarized PC12 cells enhance the resolution for immunofluorescence microscopic mapping of protein localization in the early biosynthetic pathway. The authors also describe here a rapid cell
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