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文献和实验Live Imaging of Primitive Endoderm Precursors in the Mouse Blastocyst
are still not very well understood. Recently, the identification of platelet derived growth factor receptor alpha (Pdgfrα) as an early-expressed protein that is also a marker of the later primitive endoderm lineage, together with the availability of the PdgfraH2B–GFP mouse strain
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
screening on 74 clones derived from the mouse in panel A. Notice the high specificity for the target antigen (RGS19) over MBP or RGS4‐MBP fusion protein. This method allows a large number of hybridomas (>200) to be screened in a single day for antigenic
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides fused to either a minor or major capsid protein. Direct affinity selection uses target protein bound to a microtiter plate followed
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