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文献和实验CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS
for this protocol. Transfect 2-T150s of HEK293 cells with the appropriate expression vectors (protein A and protein B) as per standard protocol. Alternatively, you can use an endogenous cell line that expressed both proteins if you have specific antibodies
Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5-Untranslated Region
7B2 is a chaperone for the prohormone/proneuropeptide convertase PC2. Its mRNA is readily detectable in most neuronal and endocrine cells; the protein, in contrast, is often found at relatively low levels, suggesting that translation
) were added. BRET measurements were recorded on the FUSION plate reader over a period of 30 min. at room temperature. BRET values in the absence of the agonist were not different from the background BRET readings. (B ) Agonist‐promoted BRET dose response. HEK293
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