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文献和实验Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
function. Many R proteins are associated with the plasma membrane (PM) and expressed at low levels. Here, we describe a method to purify such low-abundance PM R protein �complexes from Arabidopsis using a biotinylated affinity tag, called the HPB tag
Western Blotting with Biotinylated Antibodies
SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate excess PBS. 3.Add 1ml boiling lysis buffer
Selection of Antibodies Against Biotinylated Antigens
, but may also be problematic because the passive adsorption on plastic at pH 9.6 is a mechanism of protein denaturation. Under these conditions, 95% of adsorbed proteins are nonfunctional (2 ,3 ) This problem is not important for a classical enzyme-linked immunosorbant assay
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