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文献和实验In this chapter, we discuss in detail two essential methods used to evaluate the interaction of Myc with another protein of interest: co-immunoprecipitation (Co-IP) and in vitro pull-down assays. Co-IP is a method that, by immunoaffinity
activated. In fact, aberrantly elevated and/or deregulated activity of Myc is associated with the majority of human cancers. Several switchable mouse transgenic models have been developed and provided insights on the role of Myc in maintaining multiple
Analyzing Myc in Cell Transformation and Evolution
levels of the Myc protein is a frequent event in the etiology of human cancers. In this chapter, we describe cell systems and experimental strategies to monitor and quantify the oncogenic potential of myc alleles and to isolate and characterize
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