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文献和实验Efficient Expression of Human Aromatase (CYP19) in E. coli
Human aromatase (CYP19, P450arom) is responsible for the conversion of androgens to estrogens. In addition to the estrogen biosynthesis in gonads and adrenals in a classical endocrine manner, this enzyme is widely expressed in various tissues
Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System
Resident cells of tissues are capable of secreting an array of structurally related zinc endopeptidases known as matrix metalloproteinases (MMPs). They initiate the degradation of the surrounding macromolecules of the extracellular matrix
Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
family, MMP-2 (gelatinase A) and MMP-9 (gelatinase B), due to their potent gelatin-degrading activity. This polyacrylamide gel electrophoresis-based method can provide a reliable assessment of the type of gelatinase, relative amount, and activation status
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