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文献和实验Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
cells HEK293T and suspension HEK293-6E cells. More than 10 �g/mL yield of scFv-Fc protein can be obtained every day for one to two weeks by transient production in HEK293T cells, whereas soluble HEK293-6E cells produce more than 100 �g/mL scFv-Fc protein
. In addition, certain subclasses of rat IgG have been found to interact efficiently with human Fc receptors and the human complement system. More important perhaps in practical terms is that, when grown as an ascites, up to 30 mL of fluid containing 1–5 mg/mL
Recombinant fusion proteins incorporating experimental protein domains fused to immunoglobulin Fc regions have become widely utilized in studies of protein–ligand interactions. The advantages of these systems include an inherent increase
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