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文献和实验FA Extraction of [insoluble] Amyloid beta from Mouse Brain
plates, as a precipitate forms when samples are loaded neat. If analyzing tissue with a lot of plaques, you need larger dilution factors to analyze amyloid beta levels within the range of the standard curve. To determine amyloid beta [c] in samples
1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
diabetic mouse model. Furthermore, the detection of peripheral autoreactive CD8+ T cells in this mouse model is indicative of beta cell killing and correlates with the development of diabetes. We describe two protocols that are helpful for the detection
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